根据阿n the protein yield target, the ExpiCHO Expression System offers three different expression protocols for maximal flexibility:

Standard Protocol: Addition of ExpiFectamine CHO Enhancer and single feed on day 1 post-transfection; maintain cells at 37 °C throughout the expression run. Using this protocol, researchers can get up to 1 gr/L in 8-10 days.

High Titer Protocol: Addition of ExpiFectamine™ CHO Enhancer and single feed on day 1 post-transfection; shift cells to 32 °C on day 1 post-transfection. Using this protocol, researchers can get up to 2 gr/L in 10-12 days.

Max Titer Protocol: Addition of ExpiFectamine™ CHO Enhancer and first feed on day 1 followed by a second feed on day 5 post-transfection; shift cells to 32 °C on day 1 post-transfection. Using this protocol, researchers can get up to 3 gr/L in 12-14 days.

Figure 1: Example of protein yield in ExpiCHO expression system.Kinetics of antibody expression in the ExpiCHO system.