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高效液相色新文章tography (HPLC) New article below High Performance Liquid Chromatography (HPLC)

High performance liquid chromatography (HPLC) is a very powerful technique that can be used to identify, quantify or purify components of a mixture.

As shown below, HPLC uses a column filled with fine beads that form the stationary phase and a solvent (or mobile phase) that moves over the stationary phase in the column due to the pressure generated by the pump.

HPLC output is recorded as achromatogram. Compounds that interact the least with the stationary phase, move faster and have a shorter retention time. Compounds that interact the most with the stationary phase, move slower and have a longer retention time.

Diagram demonstrating the flow through an HPLC instrument. On the left, a flask with solvent represents the mobile phase. A black arrow flows from the solvent through a pump. A jar containing sample compounds A B and C is injected after the pump, and joins the black arrow that flows into a column containing stationary phase. The stationary phase column shows one blue dot moving slower than 3 green dots and 4 red dots moving fastest through the stationary phase. A black arrow flows out of the stationary phase into a detector represented by a green square with a brown rectangle in it. A dotted line connects the detector to a computer data station displaying a chromatogram with red peak first, green peak second and blue peak last. Finally a black arrow from the detector flows into a waster beaker.

Figure 1.Components of an HPLC instrument

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