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New article next to Analyse der PCR New article below Analyse der PCR

Das Ergebnis einesPCR-Experimentssind Millionen von Kopien der DNA-Region, die von denPrimernmarkiert,。死Große der amplifizierten Fragmente wird durch die Primer bestimmt. Im Bild unten siehst du zum Beispiel, dass das PCR-Produkt A 200 bp lang sein wird, das PCR-Produkt B 900 bp, C 700 bp und D 400 bp.

Um diese Fragmente sichtbar zu machen, benötigen wir weitere Techniken, wie beispielsweise dieGel-Elektrophorese.

A diagram showing four pairs of complementary DNA strands and the resulting products after PCR. The DNA fragment pairs are labelled A through D, and the fragments are all 1000 base pairs long. Each pair of DNA fragments shows two primers, a forward primer in the 3 prime to 5 prime direction and a reverse primer in the 5 prime to 3 prime direction. The primers are spaced differently in each pair, and the base pairs between the two primers are what will be included in the fragments after PCR. On the right, each fragment is shown after PCR: fragment A has a length of 200 base pairs, the forward primer is located at 200 base pairs and the reverse primer is located at 400 base pairs. Fragment B is 900 base pairs long. The forward primer is located at 50 base pairs and the reverse primer is located at 950 base pairs. Fragment C is 700 base pairs long. The forward primer is located at 20 base pairs and the reverse primer is located at 720 base pairs. Fragment D is 400 base pairs long. The forward primer is located at 220 base pairs and the reverse primer is located at 620 base pairs.

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