No sub-articles
Browse articles in this level »
New article next to Steps to perform gram-staining New article below Steps to perform gram-staining

  1. 应用crystal violeton the slide and keep it for60seconds. Wash the slide withwater.

  2. 应用iodineon the slide and keep it for60seconds. Wash the slide withwater.

  3. 应用95% ethyl alcoholon the slide and keep it for5 to 10seconds. Wash the slide withwater.

  4. Finally, applysafraninon the slide and keep it for45seconds. Wash the slide withwater.

Four stages of gram staining experiment. In the first stage, the content of the bottle with a purple substance, called crystal violet, is poured on the glass slide and left there for 60 seconds. As a result, the spherical and rod-shaped structures inside the circle, representing the microscopic view of the bacteria on the glass slide, are colored in purple. In the second stage, the content of the bottle with an orange substance, called iodine, is poured on the glass slide and left there for 60 seconds. As a result, the bacteria under the microscopic view remain purple. In the third stage, the content of the bottle with a light blue substance, called 95% ethyl alcohol, is poured on the glass slide and left there for 5 to 10 seconds. As a result, the rod-shaped bacteria under microscopic view are decolorized, and the spherical ones remain purple. In the last stage, the content of the bottle with a pink substance, called safranin, is poured on the glass slide and left there for 45 seconds. As a result, the rod-shaped bacteria under microscopic view are colored in pink, and the spherical ones remain purple. The rod-shaped bacteria are named gram-negative, and the purple ones are named gram-positive.

图1:Workflow of gram staining method

Baidu