After the first evaluation, either under the microscope or the luminescence reader, researchers can perform different tests to confirm that the transfected gene is actually being expressed in the cells. Normally, awestern blotanalysis is the first option when the goal is to produce a protein since it is an easy and rapid way to detect if genes are being expressed and subsequently translated into proteins.

However, they can also perform a gel electrophoresis and DNA sequencing, depending on the final goal of the experiment.