In the ChIP exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol to elucidate the acid stress mechanism of大肠杆菌. Will you be able to complete all the steps correctly to understand howE. colisurvive in acid stress conditions?

Following is a list of the relevant theory pages:

• Chromatin immunoprecipitation definition

• ChIP-exo definition

• Exonuclease definition

• Cell culture conditions for ChIP-exo

• Cross-linking protein-DNA in ChIP-exo

• Fragmentation

• Preparation of antibody-transcription factor complex

• Magnetic beads

• Washing procedure in ChIP-exo

• Reparation of DNA sticky ends

• DNA dA tailing

• 芯片exo-adapters

• Reverse crosslinking

• Protein removal and DNA purification in ChIP-exo procedure

• PCR enrichment

• TOPO cloning

• Sanger sequencing