基因表达单元:使用测序揭示与肥胖相关的基因

Gene Expression Unit
Time to complete course: 74 min.

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About Gene Expression Unit:使用测序揭示与肥胖相关的基因Virtual Lab Simulation

现在众所周知,肥胖与您的DNA中的基因有关,但是它们是哪个?在此模拟中,您将学习如何对肥胖猪中可能过表达的基因进行测序DNA和筛选。为此,您将必须使用最新的技术,例如下一代测序和QPCR,并执行适当的数据分析以挑出特定基因。

Identify a new gene linked to obesity

作为研究肥胖和使用猪样品作为模型的项目的一部分,学生将对信使RNA分子进行测序,以鉴定与肥胖的肥胖症中是否在肥胖猪中过表达基因。他们将通过不同的技术,定量PCR(QPCR)确认结果,并了解该基因在人类代谢中的作用。

Next Generation Sequencing and qPCR

为了对样本进行排序,学生将首先必须执行RT-PCR来从mRNA中生成cDNA,然后通过添加适当的遗传标签来准备下一代测序的样品。测序后,他们将分析数据以挑出一个可以与肥胖有关的基因。为了确认他们的发现,学生还必须设计一个具有适当控制的定量PCR(QPCR)并分析结果曲线。该模拟使学生可以在下一代测序机中看到该过程,并在很短的时间内执行两种高级技术(测序和QPCR).

发布您的数据

The discovery of a new gene which could partially explain the effect of obesity would be worth publishing in an eminent scientific journal. Will you be able to single out such a gene from your sequencing experiment?

Get Started Now

发现下一代测序分析the mRNA from pig tissues and identify a new gene linked to obesity. Confirm your results using qPCR to analyze the expression levels of your gene!

技术in Lab

  • cDNA synthesis
  • 准备下一代测序的样品
  • Next Generation Sequencing data analysis
  • qpcr

学习目标

At the end of this simulation, you will be able to…

  • Prepare samples for Next Generation Sequencing
  • Understand the principles behind the Next Generation Sequencing technique
  • 使用适当的对照进行QPCR实验

仿真功能

Length– 74 minutes
语言– English, Japanese, Spanish

Examples of Related Standards

University

生活科学与第一年至2年有关

NGSS

HS-LS3-1
HS-LS3-2

IB

Topic 1: Cell Biology

AP

单元6:基因表达和调节

Screenshots of Gene Expression Unit:使用测序揭示与肥胖相关的基因Virtual Lab Simulation

合作者

Prof. Susanna Cirera Salicio

Prof. Susanna Cirera Salicio

基础动物与兽医科学学院
Faculty of Health and Medical Sciences, University of Copenhagen

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