最近开发的Next Generation Sequencingenables us to sequence thetranscriptome在一定tissue at a specific developmental time point. RNA-seq reveals a snapshot of the RNAs present and even allows us to quantify the RNAs to determine levels ofgene expression.

The instability of RNA molecules makes it necessary toreverse transcribethe transcripts into DNA for sequencing.

To sequence the mRNA we need to perform the following steps:

1. Isolate the RNA.

2. 净化mRNA.

3. Reverse transcribe RNA to cDNA.

4. DNA modifications for NGS.

5. Cluster generation for NGS.

6. NGS.