通过培养细菌定量:Count bacteria with serial dilution

通过培养细菌定量
Time to complete course: 46 min.

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关于通过培养的细菌定量:Count bacteria with serial dilution虚拟实验室模拟

Did you know that it’s possible to count bacteria with your naked eye? In this simulation, you will learn how to quantify bacteria using the process of serial dilution.

找到一种新颖的抗生素化合物

Your colleague has just returned from an expedition to the Amazon rainforest, and has brought back a promising sample; a fungus that seems to inhibit bacterial growth. Your mission is to quantify the effect of this antibiotic compound on bacterial growth. But, you will only be able to succeed if you manage to combine smart experimental design, some simple mathematics, and proper microbial culture techniques.

基于查询的方法来量化细菌

弄清楚如何通过基于查询的学习来量化细菌细胞。您将通过反复试验来学到更多,如果您应对挑战,您将获得一些额外的问题。不用担心,您的虚拟助手One博士将准备回答您的问题,并指导您执行连续稀释所需的步骤。确保使用无菌技术避免污染细菌培养物!

Don’t waste time and agar plates

In a real lab, this experiment would take a week or more to complete, but in this simulation it only takes 30 minutes! Plate out countless agar plates in order to quantify the colonies from your serial dilutions, but don’t worry, not a single plate will be wasted. You will learn how to calculate the colony forming units per milliliter (cfu/mL) of your bacterial culture using a cfu calculator.

Quantify the bacterial growth

Last, you will incubate the bacterial culture with the antibiotic compound to quantify the effect of the compound on bacterial growth.

你会able to determine if the fungal compound can be used as a new antibiotic?

Get Started Now

Use the serial dilution technique to quantify the effect of a novel antibiotic compound on bacterial growth.

实验室中的技术

  • 连续稀释
  • 殖民地形成单位计算
  • Aseptic Technique

Learning Objectives

在此模拟结束时,您将能够…

  • 建立对细菌培养物的连续稀释,并描述为什么使用它们。
  • Calculate the cfu/ml from colonies counted on an agar plate, given the dilution factor and volume correction factor.
  • Set up an experiment and interpret your results.
  • 举例说明为什么必须测量细菌生长速率

Simulation Features

长度– 46 minutes
Accessibility mode– Not Available
Languages- 英式西班牙语

Examples of Related Standards

大学

Life sciences and Microbiology related 1st Year

NGSS

No direct alignment

IB

B.1 Microbiology: organisms in industry

AP

No direct alignment

Screenshots of Bacterial Quantification by Culture:Count bacteria with serial dilution虚拟实验室模拟

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