CRISPR-CAS应用于TGF-BETA诱导的EMT:Set up protocols in the lab to answer biological questions

CRISPR may sound like a brand of cereals, but it certainly isn’t! In this simulation, you will learn to identify the molecular hallmarks of the Epithelial-to-Mesenchymal Transition (EMT) molecular process in breast cancer cells. You will induce EMT by adding TGF-beta to the cells, and, after identifying the hallmarks with immunofluorescence labeling, you will use the cutting-edge CRISPR-Cas9 technology to try to revert this process, which is one of the main causes of cancer malignancy.

Set up your own immunofluorescence protocol

In your first mission, you will be able to perform an immunostaining protocol by making decisions on the key steps of the process. Each decision can get you closer to the correct outcome, so think carefully and choose wisely!

Use CRISPR to knock out a specific gene

一旦您了解了EMT的分子标志，您将能够潜入真核细胞，以了解CRISPR-CAS9技术如何在分子水平上起作用。然后，您将通过解决有趣的难题来学习设计CRISPR-CAS9组件之一。最后，您将选择目标基因来淘汰，您的虚拟实验室助理One博士将帮助您完成免疫染色过程的最后一部分，以便您可以直接评估结果。

Repeat your experiment as many times as you want

At the end of each mission, you will be able to repeat the experiment if the outcome was not the desired one, giving you the possibility of learning from your mistakes. Luckily, Dr. One will be there to help you with some of the tasks. In the end, you will be able to complete an experiment that takes weeks in just less than one hour!

Will you be able to set the right protocols in place, solving any issues that arise, to understand the molecular changes linked to the TGF-beta induced EMT and evaluate the possibility of using CRISPR-Cas9 as a possible therapeutic approach in breast cancer?