Polymerase Chain Reaction

Time to complete course: 33 min.

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About Polymerase Chain ReactionVirtual Lab Simulation

在聚合酶链反应(PCR)模拟中,您将被扔进发生谋杀案的犯罪现场。为了调查犯罪现场,您的第一个任务是收集血液样本,以期凶手留下其DNA的痕迹。

Analyze DNA

After sampling you will go to the lab to isolate and analyze the sample of DNA you collected. By using a PCR kit, a thermocycler, and the purified DNA from the crime scene, it is up to you to mix the correct reagents and perform the PCR experiment.

See the structure of DNA and its replication up close

A 3D animation will show the PCR experiment at the molecular level, illustrating the structure of DNA and its replication. Quiz questions will be asked throughout the experimental process, as well as at specific steps of the PCR itself.

Identify the murderer

In the PCR simulation, you will your collected sample and other prepared samples from the suspects on a gel, and then compare the patterns that emerge.

Will you be able to identify the murderer?

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Learn the techniques and application of Polymerase Chain Reaction and Gel Electrophoresis. Explore real world application, such as analyzing unique genetic fingerprints to solve a murder case.

Techniques in Lab

  • 聚合酶链反应(PCR)
  • Gel electrophoresis
  • DNA profiling

Learning Objectives

At the end of this simulation, you will be able to…

  • Explain the function of DNA polymerase in DNA replication and synthesis
  • Perform a PCR experiment using DNA from a blood sample as the template
  • Carry out a gel electrophoresis that separates DNA according to its size
  • Interpret the unique signature of the human genome and the use of tandem repeated regions (TRR) in DNA profiling

Simulation Features

Length– 33 minutes
可访问性mode– Available
Languages– English

Examples of Related Standards

University

Life Sciences related 1st and 2nd year onwards

NGSS

No direct alignment

IB

2.7 DNA replication, transcription and translation
3.5 Genetic modification and biotechnology
B.4医学

AP

No direct alignment

Screenshots of Polymerase Chain ReactionVirtual Lab Simulation

Collaborators

Dr. Mette Voldby Larsen

Dr. Mette Voldby Larsen

Institute of Systems Biology
丹麦Technical University

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