Introduction

• 抗利尿激素enzyme kinetics

Lab Theory

• Enzyme
  • 抗利尿激素
  • Active site
  • Substrate
  • Co-factor
  • Co-factor for alcohol dehydrogenase
  • 产品
• Enzyme kinetic assay
• Michaelis-Menten
  • Michealis-Menten equation
  • Reaction rate
  • Initial reaction rate
  • K_m
  • V_max
  • k_cat
• Inhibitors
  • Competitive inhibition
  • Un-competitive inhibition
  • 混合/非竞争性抑制
• Master mix
• Spectrophotometer